Laser-Driven Microsecond Temperature Cycles Analyzed by Fluorescence Polarization Microscopy

We demonstrate a novel technique to achieve fast thermal cycles of a small sample (a few femtoliters). Modulating a continuous near-infrared laser focused on a metal film, we can drive the local temperature from 130 to 300 K and back, within a few microseconds. By fluorescence microscopy of dyes in...

Full description

Saved in:
Bibliographic Details
Main Authors: Zondervan, Rob, Kulzer, Florian, van der Meer, Harmen, Disselhorst, Jos A. J. M., Orrit, Michel
Format: Artigo
Language:Inglês
Published: Biophysical Society 2006
Subjects:
Spectroscopy, Imaging, Other Techniques
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1414575/
https://ncbi.nlm.nih.gov/pubmed/16443653
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.075168
Tags: Add Tag
No Tags, Be the first to tag this record!

Similar Items