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Polarized Fluorescence Resonance Energy Transfer Microscopy

Current methods for fluorescence resonance energy transfer (FRET) microscopy of living cells involve taking a series of images with alternating excitation colors in separate camera exposures. Here we present a new FRET method based on polarization that requires only one camera exposure and thereby o...

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Autors principals: Mattheyses, Alexa L., Hoppe, Adam D., Axelrod, Daniel
Format: Artigo
Idioma:Inglês
Publicat: Biophysical Society 2004
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC1304697/
https://ncbi.nlm.nih.gov/pubmed/15454470
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.103.036194
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