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Measurement of Single Macromolecule Orientation by Total Internal Reflection Fluorescence Polarization Microscopy
A new approach is presented for measuring the three-dimensional orientation of individual macromolecules using single molecule fluorescence polarization (SMFP) microscopy. The technique uses the unique polarizations of evanescent waves generated by total internal reflection to excite the dipole mome...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Biophysical Society
2005
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1366610/ https://ncbi.nlm.nih.gov/pubmed/15894632 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.053470 |
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