Orientation of the Myosin Light Chain Region by Single Molecule Total Internal Reflection Fluorescence Polarization Microscopy

To study the orientation and dynamics of myosin, we measured fluorescence polarization of single molecules and ensembles of myosin decorating actin filaments. Engineered chicken gizzard regulatory light chain (RLC), labeled with bisiodoacetamidorhodamine at cysteine residues 100 and 108 or 104 and 1...

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Hlavní autoři: Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Médium: Artigo
Jazyk:Inglês
Vydáno: Biophysical Society 2005
Témata:
Muscle and Contractility
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366598/
https://ncbi.nlm.nih.gov/pubmed/15894631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.053496
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