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Orientation of the Myosin Light Chain Region by Single Molecule Total Internal Reflection Fluorescence Polarization Microscopy

To study the orientation and dynamics of myosin, we measured fluorescence polarization of single molecules and ensembles of myosin decorating actin filaments. Engineered chicken gizzard regulatory light chain (RLC), labeled with bisiodoacetamidorhodamine at cysteine residues 100 and 108 or 104 and 1...

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Bibliografski detalji
Glavni autori: Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Format: Artigo
Jezik:Inglês
Izdano: Biophysical Society 2005
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC1366598/
https://ncbi.nlm.nih.gov/pubmed/15894631
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.104.053496
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