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The Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Twirling Filament Assay

Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single molecules. polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule o...

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Bibliografiske detaljer
Main Authors: Beausang, John F., Sun, Yujie, Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3852181/
https://ncbi.nlm.nih.gov/pubmed/22661429
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot069401
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