Construction of Flow Chambers for Polarized Total Internal Reflection Fluorescence Microscopy (polTIRFM) Motility Assays

Polarized total internal reflection fluorescence microscopy (polTIRFM) can be used to detect the spatial orientation and rotational dynamics of single molecules. polTIRFM determines the three-dimensional angular orientation and the extent of wobble of a fluorescent probe bound to the macromolecule o...

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Main Authors: Beausang, John F., Sun, Yujie, Quinlan, Margot E., Forkey, Joseph N., Goldman, Yale E.
Formato: Artigo
Idioma:Inglês
Publicado: 2012
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Article
Acceso en liña:https://ncbi.nlm.nih.gov/pmc/articles/PMC3855857/
https://ncbi.nlm.nih.gov/pubmed/22661445
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/pdb.prot069385
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