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Laser-Driven Microsecond Temperature Cycles Analyzed by Fluorescence Polarization Microscopy
We demonstrate a novel technique to achieve fast thermal cycles of a small sample (a few femtoliters). Modulating a continuous near-infrared laser focused on a metal film, we can drive the local temperature from 130 to 300 K and back, within a few microseconds. By fluorescence microscopy of dyes in...
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| Autori principali: | , , , , |
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| Natura: | Artigo |
| Lingua: | Inglês |
| Pubblicazione: |
Biophysical Society
2006
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| Soggetti: | |
| Accesso online: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1414575/ https://ncbi.nlm.nih.gov/pubmed/16443653 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1529/biophysj.105.075168 |
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