Investigating the endonuclease activity of four Pyrococcus abyssi inteins

Among the 14 inteins of the Pyrococcus abyssi genome, 10 harbour the LAGLIDADG motifs of dodecapeptide endonucleases. Four of these were cloned, expressed in Escherichia coli and purified to assay their potential endonuclease activity. PabRIR1-2 and PabRIR1-3 are specific endonucleases, named PI-Pab...

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Publié dans:	Nucleic Acids Res
Auteurs principaux:	Saves, Isabelle, Morlot, Cécile, Thion, Laurent, Rolland, Jean-Luc, Diétrich, Jacques, Masson, Jean-Michel
Format:	Artigo
Langue:	Inglês
Publié:	Oxford University Press 2002
Sujets:	Articles
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12364594/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf556
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