Investigating the endonuclease activity of four Pyrococcus abyssi inteins

Among the 14 inteins of the Pyrococcus abyssi genome, 10 harbour the LAGLIDADG motifs of dodecapeptide endonucleases. Four of these were cloned, expressed in Escherichia coli and purified to assay their potential endonuclease activity. PabRIR1-2 and PabRIR1-3 are specific endonucleases, named PI-Pab...

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Manylion Llyfryddiaeth

Cyhoeddwyd yn:	Nucleic Acids Res
Prif Awduron:	Saves, Isabelle, Morlot, Cécile, Thion, Laurent, Rolland, Jean-Luc, Diétrich, Jacques, Masson, Jean-Michel
Fformat:	Artigo
Iaith:	Inglês
Cyhoeddwyd:	Oxford University Press 2002
Pynciau:	Articles
Mynediad Ar-lein:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12364594/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf556
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