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Investigating the endonuclease activity of four Pyrococcus abyssi inteins

Among the 14 inteins of the Pyrococcus abyssi genome, 10 harbour the LAGLIDADG motifs of dodecapeptide endonucleases. Four of these were cloned, expressed in Escherichia coli and purified to assay their potential endonuclease activity. PabRIR1-2 and PabRIR1-3 are specific endonucleases, named PI-Pab...

Deskribapen osoa

Gorde:
Xehetasun bibliografikoak
Egile Nagusiak: Saves, Isabelle, Morlot, Cécile, Thion, Laurent, Rolland, Jean-Luc, Diétrich, Jacques, Masson, Jean-Michel
Formatua: Artigo
Hizkuntza:Inglês
Argitaratua: Oxford University Press 2002
Gaiak:
Sarrera elektronikoa:https://ncbi.nlm.nih.gov/pmc/articles/PMC140554/
https://ncbi.nlm.nih.gov/pubmed/12364594
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