Investigating the endonuclease activity of four Pyrococcus abyssi inteins

Among the 14 inteins of the Pyrococcus abyssi genome, 10 harbour the LAGLIDADG motifs of dodecapeptide endonucleases. Four of these were cloned, expressed in Escherichia coli and purified to assay their potential endonuclease activity. PabRIR1-2 and PabRIR1-3 are specific endonucleases, named PI-Pab...

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Dades bibliogràfiques

Publicat a:	Nucleic Acids Res
Autors principals:	Saves, Isabelle, Morlot, Cécile, Thion, Laurent, Rolland, Jean-Luc, Diétrich, Jacques, Masson, Jean-Michel
Format:	Artigo
Idioma:	Inglês
Publicat:	Oxford University Press 2002
Matèries:	Articles
Accés en línia:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC140554/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12364594/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/gkf556
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