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The Type II Pullulanase of Thermococcus hydrothermalis: Molecular Characterization of the Gene and Expression of the Catalytic Domain

The gene encoding a hyperthermostable type II pullulanase produced by Thermococcus hydrothermalis (Th-Apu) has been isolated. Analysis of a total of 5.2 kb of genomic DNA has revealed the presence of three open reading frames, one of which (apuA) encodes the pullulanase. This enzyme is composed of 1...

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Autores principales: Erra-Pujada, Marta, Debeire, Philippe, Duchiron, Francis, O’Donohue, Michael J.
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 1999
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC93789/
https://ncbi.nlm.nih.gov/pubmed/10322035
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