The Type II Pullulanase of Thermococcus hydrothermalis: Molecular Characterization of the Gene and Expression of the Catalytic Domain

The gene encoding a hyperthermostable type II pullulanase produced by Thermococcus hydrothermalis (Th-Apu) has been isolated. Analysis of a total of 5.2 kb of genomic DNA has revealed the presence of three open reading frames, one of which (apuA) encodes the pullulanase. This enzyme is composed of 1...

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Pubblicato in:	J Bacteriol
Autori principali:	Erra-Pujada, Marta, Debeire, Philippe, Duchiron, Francis, O’Donohue, Michael J.
Natura:	Artigo
Lingua:	Inglês
Pubblicazione:	American Society for Microbiology (ASM) 1999
Soggetti:	Enzymes and Proteins
Accesso online:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC93789/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10322035/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/jb.181.10.3284-3287.1999
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