Efficient fluorescence labeling of a large RNA through oligonucleotide hybridization

We present an efficient method of introducing fluorophore labels at selected locations in a large RNA. The method is based on specific and highly efficient hybridization between a fluorophore-containing DNA oligonucleotide and a modular hairpin loop replacing a functionally unimportant hairpin loop...

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Detalhes bibliográficos
Main Authors: SMITH, GLENNA J., SOSNICK, TOBIN R., SCHERER, NORBERT F., PAN, TAO
Formato: Artigo
Idioma:Inglês
Publicado em: Copyright 2005 by RNA Society 2005
Assuntos:
Method
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370712/
https://ncbi.nlm.nih.gov/pubmed/15613536
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7180305
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