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Efficient fluorescence labeling of a large RNA through oligonucleotide hybridization
We present an efficient method of introducing fluorophore labels at selected locations in a large RNA. The method is based on specific and highly efficient hybridization between a fluorophore-containing DNA oligonucleotide and a modular hairpin loop replacing a functionally unimportant hairpin loop...
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| Hauptverfasser: | , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
Copyright 2005 by RNA Society
2005
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370712/ https://ncbi.nlm.nih.gov/pubmed/15613536 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7180305 |
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