Unlabeled Helper Oligonucleotides Increase the In Situ Accessibility to 16S rRNA of Fluorescently Labeled Oligonucleotide Probes

Target site inaccessibility represents a significant problem for fluorescence in situ hybridization (FISH) of 16S rRNA with oligonucleotide probes. Here, unlabeled oligonucleotides (helpers) that bind adjacent to the probe target site were evaluated for their potential to increase weak probe hybridi...

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Библиографические подробности
Главные авторы: Fuchs, Bernhard M., Glöckner, Frank Oliver, Wulf, Jörg, Amann, Rudolf
Формат: Artigo
Язык:Inglês
Опубликовано: American Society for Microbiology 2000
Предметы:
Methods
Online-ссылка:https://ncbi.nlm.nih.gov/pmc/articles/PMC92190/
https://ncbi.nlm.nih.gov/pubmed/10919826
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