Unlabeled Helper Oligonucleotides Increase the In Situ Accessibility to 16S rRNA of Fluorescently Labeled Oligonucleotide Probes

Target site inaccessibility represents a significant problem for fluorescence in situ hybridization (FISH) of 16S rRNA with oligonucleotide probes. Here, unlabeled oligonucleotides (helpers) that bind adjacent to the probe target site were evaluated for their potential to increase weak probe hybridi...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Fuchs, Bernhard M., Glöckner, Frank Oliver, Wulf, Jörg, Amann, Rudolf
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2000
Assuntos:	Methods
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC92190/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/10919826/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.66.8.3603-3607.2000
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