Flow Cytometric Analysis of the In Situ Accessibility of Escherichia coli 16S rRNA for Fluorescently Labeled Oligonucleotide Probes

In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure...

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Main Authors: Fuchs, Bernhard Maximilian, Wallner, Günter, Beisker, Wolfgang, Schwippl, Ines, Ludwig, Wolfgang, Amann, Rudolf
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology 1998
Teme:
General Microbial Ecology
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC90951/
https://ncbi.nlm.nih.gov/pubmed/9835591
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