Flow Cytometric Analysis of the In Situ Accessibility of Escherichia coli 16S rRNA for Fluorescently Labeled Oligonucleotide Probes

In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Fuchs, Bernhard Maximilian, Wallner, Günter, Beisker, Wolfgang, Schwippl, Ines, Ludwig, Wolfgang, Amann, Rudolf
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 1998
Assuntos:	General Microbial Ecology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90951/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835591/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.4973-4982.1998
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