Flow Cytometric Analysis of the In Situ Accessibility of Escherichia coli 16S rRNA for Fluorescently Labeled Oligonucleotide Probes

In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure...

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Detalles Bibliográficos

Publicado en:	Appl Environ Microbiol
Autores principales:	Fuchs, Bernhard Maximilian, Wallner, Günter, Beisker, Wolfgang, Schwippl, Ines, Ludwig, Wolfgang, Amann, Rudolf
Formato:	Artigo
Lenguaje:	Inglês
Publicado:	American Society for Microbiology (ASM) 1998
Materias:	General Microbial Ecology
Acceso en línea:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90951/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835591/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.4973-4982.1998
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