Flow Cytometric Analysis of the In Situ Accessibility of Escherichia coli 16S rRNA for Fluorescently Labeled Oligonucleotide Probes

In situ identification of whole fixed bacterial cells by hybridization with fluorescently labeled, rRNA-targeted oligonucleotide probes is often limited by low signal intensities. In addition to an impermeability of the cell periphery and a low cellular rRNA content, the three-dimensional structure...

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Bibliografische gegevens

Gepubliceerd in:	Appl Environ Microbiol
Hoofdauteurs:	Fuchs, Bernhard Maximilian, Wallner, Günter, Beisker, Wolfgang, Schwippl, Ines, Ludwig, Wolfgang, Amann, Rudolf
Formaat:	Artigo
Taal:	Inglês
Gepubliceerd in:	American Society for Microbiology (ASM) 1998
Onderwerpen:	General Microbial Ecology
Online toegang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC90951/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9835591/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/aem.64.12.4973-4982.1998
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