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Fluorescent-labeled oligonucleotide probes: detection of hybrid formation in solution by fluorescence polarization spectroscopy.

Fluorescein-labeled oligonucleotides as DNA-probes were synthesized and used to monitor hybrid formation, namely to detect DNA or oligonucleotide sequence in solution. The introduction of fluorescein to oligonucleotides was carried out by oxidation of a hydrogen phosphonate linkage with ethylenediam...

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Bibliographische Detailangaben
Hauptverfasser: Murakami, A, Nakaura, M, Nakatsuji, Y, Nagahara, S, Tran-Cong, Q, Makino, K
Format: Artigo
Sprache:Inglês
Veröffentlicht: 1991
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC328546/
https://ncbi.nlm.nih.gov/pubmed/1870966
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