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Fluorescent-labeled oligonucleotide probes: detection of hybrid formation in solution by fluorescence polarization spectroscopy.
Fluorescein-labeled oligonucleotides as DNA-probes were synthesized and used to monitor hybrid formation, namely to detect DNA or oligonucleotide sequence in solution. The introduction of fluorescein to oligonucleotides was carried out by oxidation of a hydrogen phosphonate linkage with ethylenediam...
Shranjeno v:
| Main Authors: | , , , , , |
|---|---|
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
1991
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| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC328546/ https://ncbi.nlm.nih.gov/pubmed/1870966 |
| Oznake: |
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