Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

Fluorescence in situ hybridization (FISH) with singly labeled rRNA-targeted oligonucleotide probes is widely applied for direct identification of microbes in the environment or in clinical specimens. Here we show that a replacement of singly labeled oligonucleotide probes with 5′-, 3′-doubly labeled...

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Detaylı Bibliyografya
Asıl Yazarlar: Stoecker, Kilian, Dorninger, Christiane, Daims, Holger, Wagner, Michael
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology (ASM) 2010
Konular:
Methods
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC2813028/
https://ncbi.nlm.nih.gov/pubmed/19966029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02456-09
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