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Double Labeling of Oligonucleotide Probes for Fluorescence In Situ Hybridization (DOPE-FISH) Improves Signal Intensity and Increases rRNA Accessibility

Fluorescence in situ hybridization (FISH) with singly labeled rRNA-targeted oligonucleotide probes is widely applied for direct identification of microbes in the environment or in clinical specimens. Here we show that a replacement of singly labeled oligonucleotide probes with 5′-, 3′-doubly labeled...

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Bibliografski detalji
Glavni autori: Stoecker, Kilian, Dorninger, Christiane, Daims, Holger, Wagner, Michael
Format: Artigo
Jezik:Inglês
Izdano: American Society for Microbiology (ASM) 2010
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2813028/
https://ncbi.nlm.nih.gov/pubmed/19966029
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02456-09
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