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Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based assay

Ribonuclease P (RNase P) is a ribonucleoprotein complex that catalyzes the cleavage of the 5′ end of precursor tRNA. To characterize the interface between the Bacillus subtilis RNA (PRNA) and protein (P protein) components, the intraholoenzyme K(D) is determined as a function of ionic strength using...

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Main Authors: DAY-STORMS, JEREMY J., NIRANJANAKUMARI, S., FIERKE, CAROL A.
格式: Artigo
語言:Inglês
出版: Copyright 2004 by RNA Society 2004
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370646/
https://ncbi.nlm.nih.gov/pubmed/15337847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7550104
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