Ionic interactions between PRNA and P protein in Bacillus subtilis RNase P characterized using a magnetocapture-based assay

Ribonuclease P (RNase P) is a ribonucleoprotein complex that catalyzes the cleavage of the 5′ end of precursor tRNA. To characterize the interface between the Bacillus subtilis RNA (PRNA) and protein (P protein) components, the intraholoenzyme K(D) is determined as a function of ionic strength using...

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Bibliographic Details
Main Authors: DAY-STORMS, JEREMY J., NIRANJANAKUMARI, S., FIERKE, CAROL A.
Format: Artigo
Language:Inglês
Published: Copyright 2004 by RNA Society 2004
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Article
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370646/
https://ncbi.nlm.nih.gov/pubmed/15337847
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7550104
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