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Detection and quantitation of RNA base modifications

Using a new combination of previously published techniques, we developed a method for quantitating modified nucleotides in RNAs. First, an RNA is cleaved with RNase H at the 5′ side of a nucleotide of interest. Next, (32)P is substituted for the phosphate at the 5′ end of this nucleotide. Finally, a...

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Autori principali: ZHAO, XINLIANG, YU, YI-TAO
Natura: Artigo
Lingua:Inglês
Pubblicazione: Copyright 2004 by RNA Society 2004
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC1370591/
https://ncbi.nlm.nih.gov/pubmed/15146083
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7110804
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