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Detection and quantitation of RNA base modifications
Using a new combination of previously published techniques, we developed a method for quantitating modified nucleotides in RNAs. First, an RNA is cleaved with RNase H at the 5′ side of a nucleotide of interest. Next, (32)P is substituted for the phosphate at the 5′ end of this nucleotide. Finally, a...
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| Main Authors: | , |
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| 格式: | Artigo |
| 語言: | Inglês |
| 出版: |
Copyright 2004 by RNA Society
2004
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| 主題: | |
| 在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1370591/ https://ncbi.nlm.nih.gov/pubmed/15146083 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.7110804 |
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