Identification of Eukaryotic Open Reading Frames in Metagenomic cDNA Libraries Made from Environmental Samples

Here we describe the application of metagenomic technologies to construct cDNA libraries from RNA isolated from environmental samples. RNAlater (Ambion) was shown to stabilize RNA in environmental samples for periods of at least 3 months at −20°C. Protocols for library construction were established...

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Bibliographic Details
Main Authors: Grant, Susan, Grant, William D., Cowan, Don A., Jones, Brian E., Ma, Yanhe, Ventosa, Antonio, Heaphy, Shaun
Format: Artigo
Language:Inglês
Published: American Society for Microbiology 2006
Subjects:
Methods
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC1352286/
https://ncbi.nlm.nih.gov/pubmed/16391035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.1.135-143.2006
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