Identification of Eukaryotic Open Reading Frames in Metagenomic cDNA Libraries Made from Environmental Samples

Here we describe the application of metagenomic technologies to construct cDNA libraries from RNA isolated from environmental samples. RNAlater (Ambion) was shown to stabilize RNA in environmental samples for periods of at least 3 months at −20°C. Protocols for library construction were established...

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Detaylı Bibliyografya
Asıl Yazarlar: Grant, Susan, Grant, William D., Cowan, Don A., Jones, Brian E., Ma, Yanhe, Ventosa, Antonio, Heaphy, Shaun
Materyal Türü: Artigo
Dil:Inglês
Baskı/Yayın Bilgisi: American Society for Microbiology 2006
Konular:
Methods
Online Erişim:https://ncbi.nlm.nih.gov/pmc/articles/PMC1352286/
https://ncbi.nlm.nih.gov/pubmed/16391035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.1.135-143.2006
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