Identification of Eukaryotic Open Reading Frames in Metagenomic cDNA Libraries Made from Environmental Samples

Here we describe the application of metagenomic technologies to construct cDNA libraries from RNA isolated from environmental samples. RNAlater (Ambion) was shown to stabilize RNA in environmental samples for periods of at least 3 months at −20°C. Protocols for library construction were established...

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Detalhes bibliográficos
Main Authors: Grant, Susan, Grant, William D., Cowan, Don A., Jones, Brian E., Ma, Yanhe, Ventosa, Antonio, Heaphy, Shaun
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology 2006
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC1352286/
https://ncbi.nlm.nih.gov/pubmed/16391035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.1.135-143.2006
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