Identification of Eukaryotic Open Reading Frames in Metagenomic cDNA Libraries Made from Environmental Samples

Here we describe the application of metagenomic technologies to construct cDNA libraries from RNA isolated from environmental samples. RNAlater (Ambion) was shown to stabilize RNA in environmental samples for periods of at least 3 months at −20°C. Protocols for library construction were established...

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Detalles Bibliográficos
Autores principales: Grant, Susan, Grant, William D., Cowan, Don A., Jones, Brian E., Ma, Yanhe, Ventosa, Antonio, Heaphy, Shaun
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Microbiology 2006
Materias:
Methods
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC1352286/
https://ncbi.nlm.nih.gov/pubmed/16391035
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.72.1.135-143.2006
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