A Human cDNA Expression Library in Yeast Enriched for Open Reading Frames

We developed a high-throughput technique for the generation of cDNA libraries in the yeast Saccharomyces cerevisiae which enables the selection of cloned cDNA inserts containing open reading frames (ORFs). For direct screening of random-primed cDNA libraries, we have constructed a yeast shuttle/expr...

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Detalhes bibliográficos
Main Authors: Holz, Caterina, Lueking, Angelika, Bovekamp, Lara, Gutjahr, Claudia, Bolotina, Natalia, Lehrach, Hans, Cahill, Dolores J.
Formato: Artigo
Idioma:Inglês
Publicado em: Cold Spring Harbor Laboratory Press 2001
Assuntos:
Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC311161/
https://ncbi.nlm.nih.gov/pubmed/11591650
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1101/gr.181501
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