Engineering of Polyploid Saccharomyces cerevisiae for Secretion of Large Amounts of Fungal Glucoamylase

We engineered Saccharomyces cerevisiae cells that produce large amounts of fungal glucoamylase (GAI) from Aspergillus awamori var. kawachi. To do this, we used the δ-sequence-mediated integration vector system and the heat-induced endomitotic diploidization method. δ-Sequence-mediated integration is...

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Detalhes bibliográficos

Publicado no:	Appl Environ Microbiol
Principais autores:	Ekino, Keisuke, Hayashi, Hiroyuki, Moriyama, Masahiro, Matsuda, Minoru, Goto, Masatoshi, Yoshino, Sadazo, Furukawa, Kensuke
Formato:	Artigo
Idioma:	Inglês
Publicado em:	American Society for Microbiology (ASM) 2002
Assuntos:	Physiology and Biotechnology
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406766/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5693-5697.2002
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