Engineering of Polyploid Saccharomyces cerevisiae for Secretion of Large Amounts of Fungal Glucoamylase

We engineered Saccharomyces cerevisiae cells that produce large amounts of fungal glucoamylase (GAI) from Aspergillus awamori var. kawachi. To do this, we used the δ-sequence-mediated integration vector system and the heat-induced endomitotic diploidization method. δ-Sequence-mediated integration is...

Description complète

Détails bibliographiques

Publié dans:	Appl Environ Microbiol
Auteurs principaux:	Ekino, Keisuke, Hayashi, Hiroyuki, Moriyama, Masahiro, Matsuda, Minoru, Goto, Masatoshi, Yoshino, Sadazo, Furukawa, Kensuke
Format:	Artigo
Langue:	Inglês
Publié:	American Society for Microbiology (ASM) 2002
Sujets:	Physiology and Biotechnology
Accès en ligne:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406766/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5693-5697.2002
Tags:	Ajouter un tag Pas de tags, Soyez le premier à ajouter un tag!