Engineering of Polyploid Saccharomyces cerevisiae for Secretion of Large Amounts of Fungal Glucoamylase

We engineered Saccharomyces cerevisiae cells that produce large amounts of fungal glucoamylase (GAI) from Aspergillus awamori var. kawachi. To do this, we used the δ-sequence-mediated integration vector system and the heat-induced endomitotic diploidization method. δ-Sequence-mediated integration is...

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Detaylı Bibliyografya
Yayımlandı:	Appl Environ Microbiol
Asıl Yazarlar:	Ekino, Keisuke, Hayashi, Hiroyuki, Moriyama, Masahiro, Matsuda, Minoru, Goto, Masatoshi, Yoshino, Sadazo, Furukawa, Kensuke
Materyal Türü:	Artigo
Dil:	Inglês
Baskı/Yayın Bilgisi:	American Society for Microbiology (ASM) 2002
Konular:	Physiology and Biotechnology
Online Erişim:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406766/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5693-5697.2002
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Engineering of Polyploid Saccharomyces cerevisiae for Secretion of Large Amounts of Fungal Glucoamylase

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