Engineering of Polyploid Saccharomyces cerevisiae for Secretion of Large Amounts of Fungal Glucoamylase

We engineered Saccharomyces cerevisiae cells that produce large amounts of fungal glucoamylase (GAI) from Aspergillus awamori var. kawachi. To do this, we used the δ-sequence-mediated integration vector system and the heat-induced endomitotic diploidization method. δ-Sequence-mediated integration is...

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Bibliografiske detaljer

Udgivet i:	Appl Environ Microbiol
Principais autores:	Ekino, Keisuke, Hayashi, Hiroyuki, Moriyama, Masahiro, Matsuda, Minoru, Goto, Masatoshi, Yoshino, Sadazo, Furukawa, Kensuke
Format:	Artigo
Sprog:	Inglês
Udgivet:	American Society for Microbiology (ASM) 2002
Fag:	Physiology and Biotechnology
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC129939/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12406766/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/AEM.68.11.5693-5697.2002
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