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Evidence against stabilization of the transition state oxyanion by a pKa-perturbed RNA base in the peptidyl transferase center

The crystal structure of the ribosomal 50S subunit from Haloarcula marismortui in complex with the transition state analog CCdA-phosphate-puromycin (CCdApPmn) led to a mechanistic proposal wherein the universally conversed A2451 in the ribosomal active site acts as an “oxyanion hole” to promote the...

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Detalhes bibliográficos
Main Authors: Parnell, K. Mark, Seila, Amy C., Strobel, Scott A.
Formato: Artigo
Idioma:Inglês
Publicado em: The National Academy of Sciences 2002
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC129325/
https://ncbi.nlm.nih.gov/pubmed/12185248
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.182210099
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