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Cell-free cloning using φ29 DNA polymerase
We describe conditions for rolling-circle amplification (RCA) of individual DNA molecules 5–7 kb in size by >10(9)-fold, using φ29 DNA polymerase. The principal difficulty with amplification of small amounts of template by RCA using φ29 DNA polymerase is “background” DNA synthesis that usually oc...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2005
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| Teme: | |
| Online dostop: | https://ncbi.nlm.nih.gov/pmc/articles/PMC1283157/ https://ncbi.nlm.nih.gov/pubmed/16286637 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0508809102 |
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