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Generating a synthetic genome by whole genome assembly: φX174 bacteriophage from synthetic oligonucleotides
We have improved upon the methodology and dramatically shortened the time required for accurate assembly of 5- to 6-kb segments of DNA from synthetic oligonucleotides. As a test of this methodology, we have established conditions for the rapid (14-day) assembly of the complete infectious genome of b...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
2003
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307586/ https://ncbi.nlm.nih.gov/pubmed/14657399 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.2237126100 |
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