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Improvement of φ29 DNA polymerase amplification performance by fusion of DNA binding motifs

Bacteriophage φ29 DNA polymerase is a unique enzyme endowed with two distinctive properties, high processivity and faithful polymerization coupled to strand displacement, that have led to the development of protocols to achieve isothermal amplification of limiting amounts of both circular plasmids a...

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Main Authors: de Vega, Miguel, Lázaro, José M., Mencía, Mario, Blanco, Luis, Salas, Margarita
Format: Artigo
Jezik:Inglês
Izdano: National Academy of Sciences 2010
Teme:
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2944734/
https://ncbi.nlm.nih.gov/pubmed/20823261
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1011428107
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