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Terminal protein-primed amplification of heterologous DNA with a minimal replication system based on phage Φ29

The DNA amplification performed by terminal protein-primed replication systems has not yet been developed for its general use to produce high amounts of DNA linked to terminal protein (TP). Here we present a method to amplify in vitro heterologous DNAs using the Φ29 DNA replication machinery and pro...

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Bibliographische Detailangaben
Hauptverfasser: Mencía, Mario, Gella, Pablo, Camacho, Ana, de Vega, Miguel, Salas, Margarita
Format: Artigo
Sprache:Inglês
Veröffentlicht: National Academy of Sciences 2011
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Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3219123/
https://ncbi.nlm.nih.gov/pubmed/22065756
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1114397108
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