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Development of a Whole-Cell Assay for Peptidoglycan Biosynthesis Inhibitors
Osmotically stabilized Escherichia coli cells subjected to freezing and thawing were utilized as the source of enzymes for a peptidoglycan pathway assay that can be used to simultaneously test all targets of the committed steps of cell wall biosynthesis. The use of (14)C-labeled UDP-N-acetylglucosam...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
American Society for Microbiology
2002
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC127110/ https://ncbi.nlm.nih.gov/pubmed/11897573 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.46.4.943-946.2002 |
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