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Development of a Whole-Cell Assay for Peptidoglycan Biosynthesis Inhibitors

Osmotically stabilized Escherichia coli cells subjected to freezing and thawing were utilized as the source of enzymes for a peptidoglycan pathway assay that can be used to simultaneously test all targets of the committed steps of cell wall biosynthesis. The use of (14)C-labeled UDP-N-acetylglucosam...

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Sonraí Bibleagrafaíochta
Main Authors: Barbosa, Maria D. F. S., Yang, Gaoyun, Fang, Jie, Kurilla, Michael G., Pompliano, David L.
Formáid: Artigo
Teanga:Inglês
Foilsithe: American Society for Microbiology 2002
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC127110/
https://ncbi.nlm.nih.gov/pubmed/11897573
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.46.4.943-946.2002
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