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RNA interference of a trypanosome topoisomerase II causes progressive loss of mitochondrial DNA

We studied the function of a Trypanosoma brucei topoisomerase II using RNA interference (RNAi). Expression of a topoisomerase II double-stranded RNA as a stem–loop caused specific degradation of mRNA followed by loss of protein. After 6 days of RNAi, the parasites’ growth rate declined and the cells...

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Autori principali: Wang, Zefeng, Englund, Paul T.
Natura: Artigo
Lingua:Inglês
Pubblicazione: Oxford University Press 2001
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC125608/
https://ncbi.nlm.nih.gov/pubmed/11532932
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/20.17.4674
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