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Glycolysis modulates trypanosome glycoprotein expression as revealed by an RNAi library
RNA interference (RNAi) is a powerful tool for identifying gene function in Trypanosoma brucei. We generated an RNAi library, the first of its kind in any organism, by ligation of genomic fragments into the vector pZJMβ. After transfection at ∼5-fold genome coverage, trypanosomes were induced to exp...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2002
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC125414/ https://ncbi.nlm.nih.gov/pubmed/12198145 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/emboj/cdf474 |
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