Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking

Intermolecular thiol cross-linking was used to determine surface-exposed positions in 250 lactose permease mutants containing single-Cys replacements in each transmembrane helix. Significant cross-linking of monomers to produce homodimers is observed in nine mutants with a 5-Å-long cross-linking age...

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Vydáno v:	Proc Natl Acad Sci U S A
Hlavní autoři:	Guan, Lan, Murphy, Franklin D., Kaback, H. Ronald
Médium:	Artigo
Jazyk:	Inglês
Vydáno:	National Academy of Sciences 2002
Témata:	Biological Sciences
On-line přístup:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC122548/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/11904412/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.052703699
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