The substrate-binding site in the lactose permease of Escherichia coli
Site-directed N-ethylmaleimide labeling was studied with Glu-126 and/or Arg-144 mutants in lactose permease containing a single, native Cys residue at position 148 in the substrate-binding site. Replacement of either Glu-126 or Arg-144 with Ala markedly decreases Cys-148 reactivity, whereas intercha...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1998
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC21417/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/9707556/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.95.17.9802 |
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