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Surface-exposed positions in the transmembrane helices of the lactose permease of Escherichia coli determined by intermolecular thiol cross-linking

Intermolecular thiol cross-linking was used to determine surface-exposed positions in 250 lactose permease mutants containing single-Cys replacements in each transmembrane helix. Significant cross-linking of monomers to produce homodimers is observed in nine mutants with a 5-Å-long cross-linking age...

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Autori principali: Guan, Lan, Murphy, Franklin D., Kaback, H. Ronald
Natura: Artigo
Lingua:Inglês
Pubblicazione: The National Academy of Sciences 2002
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC122548/
https://ncbi.nlm.nih.gov/pubmed/11904412
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.052703699
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