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Changing the lactose permease of Escherichia coli into a galactose-specific symporter
N-ethylmaleimide (NEM) modification of a lactose permease mutant containing a single-Cys in place of Ala-122 (helix IV) abolishes active lactose transport. Moreover, lactose, melibiose, and β,d-galactopyranosyl 1-thio-β,d-galactopyranoside protect against NEM inactivation of lactose transport and/or...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
The National Academy of Sciences
2002
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC124451/ https://ncbi.nlm.nih.gov/pubmed/12011425 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.102178299 |
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