Changing the lactose permease of Escherichia coli into a galactose-specific symporter
N-ethylmaleimide (NEM) modification of a lactose permease mutant containing a single-Cys in place of Ala-122 (helix IV) abolishes active lactose transport. Moreover, lactose, melibiose, and β,d-galactopyranosyl 1-thio-β,d-galactopyranoside protect against NEM inactivation of lactose transport and/or...
Spremljeno u:
| Izdano u: | Proc Natl Acad Sci U S A |
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| Glavni autori: | , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
National Academy of Sciences
2002
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| Teme: | |
| Online pristup: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC124451/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/12011425/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.102178299 |
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